Rapid Identification of Diaporthe citri by Gene Sequence Analysis

Citrus melanoses caused by <i>Diaporthe citri</i>, has been one of the serious diseases in many citrus orchards Jeju Island. To protect melanose farms, a fast and exact diagnosis method is necessary. In this study, diseased leaves dieback twigs were collected from total 49 farms within March to April 2022. A 465 fungal isolates obtained 358 isolated plant samples. Among these isolates, 40 representatives <i>D. citrii</i> which 22 18 on 23 found based cultural characteristics potato dextrose agar conidial morphology. Additionally, molecular assay was carried out compared with those morphological diagnosis. All identified as analyzing sequences at internal transcribed spacer (ITS) rDNA region using primers ITS1/ITS4 or β-tubulin primer Btdcitri-F/R. Therefore, present where results identification type consistent DNA sequence analysis certain gene, choosing suitable for suggested.